Composite

Part:BBa_K4773013:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


J23100+RBS+ycnE+RBS+FMO+Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts.Term (BBa_B0015) was chosen because it is a double terminator, which can effectively terminate transcription and protect mRNA from degradation.


Source

FMO: From the genomic sequence of Pseudomonas putida.

References